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cy7 nhs ester  (MedChemExpress)


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    Structured Review

    MedChemExpress cy7 nhs ester
    ( A ) Scheme of SH-EM-NP/EPI preparation. ( B ) Sizes and zeta potential of PLGA-NP/EPI, EM-NP/EPI, and SH-EM-NP/EPI. Data are presented as the means ± SD ( n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. *** P < 0.001; **** P < 0.0001. ( C ) A representative image of SH-EM-NP/EPI examined by TEM. Scale bar, 50 nm. ( D ) Free thiol content of nanoparticles with and without SH modification measured by the DTNB (Ellman’s reagent) assay ( n = 3). ( E ) Schematic diagram of SH-EM-NP/EPI labeled with <t>Cy7-Mal.</t> ( F ) Fluorescence images and corresponding mean fluorescence intensities of EM-NP/EPI and SH-EM-NP/EPI after 2 hours of incubation with Cy7-Mal, followed by centrifugation, washing, and resuspension ( n = 3). ( G ) The heatmap presents the abundance profiles of the top 50 high-abundance membrane proteins across the three independent SH-EM-NP/EPI sample batches. ( H ) Photos of SH-EM-NP/EPI lyophilized powder (left) and reconstituted solution (right). All samples were stored in nitrogen atmosphere. ( I ) Size of freshly prepared solution (FS) and reconstituted solution (RS) ( n = 3). ( J ) Free thiol content of FS and RS ( n = 3). In (D), (F), (I), and (J), the data are presented as the means ± SD. Statistical analysis was performed using two-tailed Student’s t test. **** P < 0.0001; n.s., not significant.
    Cy7 Nhs Ester, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy7+nhs+ester/Cy7+NHS+ester/pmc13082320-292-5-13
    Average 94 stars, based on 2 article reviews
    cy7 nhs ester - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer"

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer

    Journal: Science Advances

    doi: 10.1126/sciadv.aeb9764

    ( A ) Scheme of SH-EM-NP/EPI preparation. ( B ) Sizes and zeta potential of PLGA-NP/EPI, EM-NP/EPI, and SH-EM-NP/EPI. Data are presented as the means ± SD ( n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. *** P < 0.001; **** P < 0.0001. ( C ) A representative image of SH-EM-NP/EPI examined by TEM. Scale bar, 50 nm. ( D ) Free thiol content of nanoparticles with and without SH modification measured by the DTNB (Ellman’s reagent) assay ( n = 3). ( E ) Schematic diagram of SH-EM-NP/EPI labeled with Cy7-Mal. ( F ) Fluorescence images and corresponding mean fluorescence intensities of EM-NP/EPI and SH-EM-NP/EPI after 2 hours of incubation with Cy7-Mal, followed by centrifugation, washing, and resuspension ( n = 3). ( G ) The heatmap presents the abundance profiles of the top 50 high-abundance membrane proteins across the three independent SH-EM-NP/EPI sample batches. ( H ) Photos of SH-EM-NP/EPI lyophilized powder (left) and reconstituted solution (right). All samples were stored in nitrogen atmosphere. ( I ) Size of freshly prepared solution (FS) and reconstituted solution (RS) ( n = 3). ( J ) Free thiol content of FS and RS ( n = 3). In (D), (F), (I), and (J), the data are presented as the means ± SD. Statistical analysis was performed using two-tailed Student’s t test. **** P < 0.0001; n.s., not significant.
    Figure Legend Snippet: ( A ) Scheme of SH-EM-NP/EPI preparation. ( B ) Sizes and zeta potential of PLGA-NP/EPI, EM-NP/EPI, and SH-EM-NP/EPI. Data are presented as the means ± SD ( n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. *** P < 0.001; **** P < 0.0001. ( C ) A representative image of SH-EM-NP/EPI examined by TEM. Scale bar, 50 nm. ( D ) Free thiol content of nanoparticles with and without SH modification measured by the DTNB (Ellman’s reagent) assay ( n = 3). ( E ) Schematic diagram of SH-EM-NP/EPI labeled with Cy7-Mal. ( F ) Fluorescence images and corresponding mean fluorescence intensities of EM-NP/EPI and SH-EM-NP/EPI after 2 hours of incubation with Cy7-Mal, followed by centrifugation, washing, and resuspension ( n = 3). ( G ) The heatmap presents the abundance profiles of the top 50 high-abundance membrane proteins across the three independent SH-EM-NP/EPI sample batches. ( H ) Photos of SH-EM-NP/EPI lyophilized powder (left) and reconstituted solution (right). All samples were stored in nitrogen atmosphere. ( I ) Size of freshly prepared solution (FS) and reconstituted solution (RS) ( n = 3). ( J ) Free thiol content of FS and RS ( n = 3). In (D), (F), (I), and (J), the data are presented as the means ± SD. Statistical analysis was performed using two-tailed Student’s t test. **** P < 0.0001; n.s., not significant.

    Techniques Used: Zeta Potential Analyzer, Modification, Labeling, Fluorescence, Incubation, Centrifugation, Membrane, Two Tailed Test

    Related Articles

    Zeta Potential Analyzer:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Modification:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Labeling:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Fluorescence:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Incubation:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Centrifugation:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Membrane:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Two Tailed Test:

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE-PEG2000-SH (2 kDa) and DSPE-PEG-NH 2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer.
    Article Snippet: DiD was purchased from Invitrogen.DiD was purchased from Invitrogen.. Cy7 maleimide (CAS: 2120392- 49- 4) and Cy7 NHS Ester (CAS: 1432019- 64- 1) were purchased from MedChemExpress LLC (New Jersey, USA).. DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).DSPE- PEG2000- SH (2 kDa) and DSPE- PEG- NH2 (2 kDa) were purchased from Ruixi biological Technology (Xi’an, China).



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    Image Search Results


    ( A ) Scheme of SH-EM-NP/EPI preparation. ( B ) Sizes and zeta potential of PLGA-NP/EPI, EM-NP/EPI, and SH-EM-NP/EPI. Data are presented as the means ± SD ( n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. *** P < 0.001; **** P < 0.0001. ( C ) A representative image of SH-EM-NP/EPI examined by TEM. Scale bar, 50 nm. ( D ) Free thiol content of nanoparticles with and without SH modification measured by the DTNB (Ellman’s reagent) assay ( n = 3). ( E ) Schematic diagram of SH-EM-NP/EPI labeled with Cy7-Mal. ( F ) Fluorescence images and corresponding mean fluorescence intensities of EM-NP/EPI and SH-EM-NP/EPI after 2 hours of incubation with Cy7-Mal, followed by centrifugation, washing, and resuspension ( n = 3). ( G ) The heatmap presents the abundance profiles of the top 50 high-abundance membrane proteins across the three independent SH-EM-NP/EPI sample batches. ( H ) Photos of SH-EM-NP/EPI lyophilized powder (left) and reconstituted solution (right). All samples were stored in nitrogen atmosphere. ( I ) Size of freshly prepared solution (FS) and reconstituted solution (RS) ( n = 3). ( J ) Free thiol content of FS and RS ( n = 3). In (D), (F), (I), and (J), the data are presented as the means ± SD. Statistical analysis was performed using two-tailed Student’s t test. **** P < 0.0001; n.s., not significant.

    Journal: Science Advances

    Article Title: Mucoadhesive tumor-penetrating nanomedicine for intravesical chemo-immunotherapy against bladder cancer

    doi: 10.1126/sciadv.aeb9764

    Figure Lengend Snippet: ( A ) Scheme of SH-EM-NP/EPI preparation. ( B ) Sizes and zeta potential of PLGA-NP/EPI, EM-NP/EPI, and SH-EM-NP/EPI. Data are presented as the means ± SD ( n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test. *** P < 0.001; **** P < 0.0001. ( C ) A representative image of SH-EM-NP/EPI examined by TEM. Scale bar, 50 nm. ( D ) Free thiol content of nanoparticles with and without SH modification measured by the DTNB (Ellman’s reagent) assay ( n = 3). ( E ) Schematic diagram of SH-EM-NP/EPI labeled with Cy7-Mal. ( F ) Fluorescence images and corresponding mean fluorescence intensities of EM-NP/EPI and SH-EM-NP/EPI after 2 hours of incubation with Cy7-Mal, followed by centrifugation, washing, and resuspension ( n = 3). ( G ) The heatmap presents the abundance profiles of the top 50 high-abundance membrane proteins across the three independent SH-EM-NP/EPI sample batches. ( H ) Photos of SH-EM-NP/EPI lyophilized powder (left) and reconstituted solution (right). All samples were stored in nitrogen atmosphere. ( I ) Size of freshly prepared solution (FS) and reconstituted solution (RS) ( n = 3). ( J ) Free thiol content of FS and RS ( n = 3). In (D), (F), (I), and (J), the data are presented as the means ± SD. Statistical analysis was performed using two-tailed Student’s t test. **** P < 0.0001; n.s., not significant.

    Article Snippet: Cy7 maleimide (CAS: 2120392-49-4) and Cy7 NHS Ester (CAS: 1432019-64-1) were purchased from MedChemExpress LLC (New Jersey, USA).

    Techniques: Zeta Potential Analyzer, Modification, Labeling, Fluorescence, Incubation, Centrifugation, Membrane, Two Tailed Test

    Characteristics of human placenta mesenchymal stem cell (hpMSC) exosomes. (A) Transmission electron microscopy showing exosome morphology. (B) Particle-sizing analysis depicting size distribution. (C) Immunoblotting results confirming CD63 and CD9 marker expression. (D) Cy7-labeled hpMSC-derived exosomes biodistribution reveals fluorescence signals in various organs over time (n = 6 mice/time point, mean ± SD; *P < 0.05 vs. 0 h). (E) Pharmacokinetics of intra-tracheally administered hpMSC-derived exosomes based on plasma Cy7-dye concentrations (n = 6 mice/time point, mean ± SD). PBS: phosphate-buffered saline, Exo: hpMSC-derived exosomes.

    Journal: Korean Journal of Anesthesiology

    Article Title: Human placental mesenchymal stem cell-derived exosomes carrying hsa-let-7i-5p mitigate lung injury in a murine model of aspiration pneumonia

    doi: 10.4097/kja.25037

    Figure Lengend Snippet: Characteristics of human placenta mesenchymal stem cell (hpMSC) exosomes. (A) Transmission electron microscopy showing exosome morphology. (B) Particle-sizing analysis depicting size distribution. (C) Immunoblotting results confirming CD63 and CD9 marker expression. (D) Cy7-labeled hpMSC-derived exosomes biodistribution reveals fluorescence signals in various organs over time (n = 6 mice/time point, mean ± SD; *P < 0.05 vs. 0 h). (E) Pharmacokinetics of intra-tracheally administered hpMSC-derived exosomes based on plasma Cy7-dye concentrations (n = 6 mice/time point, mean ± SD). PBS: phosphate-buffered saline, Exo: hpMSC-derived exosomes.

    Article Snippet: For the biodistribution assay, the third cohort of 24 mice were intra-tracheally administered hpMSC-derived exosomes labeled with Cy7 mono NHS ester (Amersham), at 1 × 108 particles/mouse, a dosage that was previously shown to mitigate endotoxemia [ – ].

    Techniques: Transmission Assay, Electron Microscopy, Western Blot, Marker, Expressing, Labeling, Derivative Assay, Fluorescence, Drug discovery, Clinical Proteomics, Saline